Top 20 Biotechnology Quiz

Top 20 Biotechnology Quiz

Biotechnology is a wide field of Biology that involves living systems and organisms to make new products beneficial for mankind. It has applications in various sectors such as medicine and agriculture.

1. Restriction enzymes were discovered by

1. Smith and Nathans
2. Alexander Fleming
3. Berg
4. None

Answer:- {A} Smith and Nathans  

2. Bacteria protect themselves from viruses by fragmenting viral DNA with

1. Ligase
2. Endonuclease
3. Exonuclease
4. Gyrase

Answer:- {B} Endonuclease  

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3. Klenow fragment is derived from

1. DNA Ligase
2. DNA Pol-I
3. DNA Pol-II
4. Reverse Transcriptase

Answer:- {B} DNA Pol-I  

4. Southern blotting is

1. Attachment of probes to DNA fragments
2. Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet
3. Comparison of DNA fragments to two sources
4. Transfer of DNA fragments to electrophoretic gel from cellulose membrane

Answer:- {B} Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet  

5. ELISA is

1. Using a radiolabeled second antibody
2. Usage of RBCs
3. Using complement-mediated cell lysis
4. Addition of substrate that is converted into a colored end product

Answer:- {D} Addition of substrate that is converted into a colored end product  

6. The Golden Rice variety is rich in

1. Vitamin C
2. Β-carotene and ferritin
3. Biotin
4. Lysine

Answer:- {B} Β-carotene and ferritin  

7. The DNA fragments have sticky ends due to

1. Endonuclease
2. Unpaired bases
3. Calcium ions
4. Free methylation

Answer:- {B} Unpaired bases  

8. Plasmids are used as cloning vectors for which of the following reasons?

1. Can be multiplied in culture
2. Self-replication in bacterial cells
3. Can be multiplied in laboratories with the help of enzymes
4. Replicate freely outside bacterial cells

Answer:- {B} Self-replication in bacterial cells  

9. The human genome project was launched in the year

1. 1980
2. 1973
3. 1990
4. 1989

Answer:- {C} 1990  

10. The vaccines prepared through recombinant DNA technology are

1. Third generation vaccines
2. First-generation vaccines
3. Second-generation vaccines
4. None

Answer:- {A} Third generation vaccines  

 

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11. Which is a genetically modified crop?

1. Bt-cotton
2. Bt-brinjal
3. Golden rice
4. All

Answer:- {D} All  

12. PCR technique was invented by

1. Karry Mullis
2. Boyer
3. Sanger
4. Cohn

Answer:- {A} Karry Mullis  

13. The first transgenic plant to be produced is

1. Brinjal
2. Tobacco
3. Rice
4. Cotton

Answer:- {B} Tobacco  

14. RNA interference helps in

1. Cell proliferation
2. Micropropagation
3. Cell defense
4. Cell differentiation

Answer:- {C} Cell defense  

15. Which of the following is the quality of improved transgenic basmati rice?

1. Gives a high yield but no characteristic aroma
2. Gives a high yield and is rich in vitamin A
3. Does not require chemical fertilizers and growth hormones
4. Resistant to insects and diseases

Answer:- {B} Gives a high yield and is rich in vitamin A  

16. The first clinical application of gene therapy over a 4 year old girl was for

1. Adenosine deaminase deficiency
2. Adenosine deficiency
3. Growth deficiency
4. Adenine deficiency

Answer:- {A} Adenosine deaminase deficiency  

17. Excision and insertion of a gene is called

1. Biotechnology
2. Genetic engineering
3. Cytogenetics
4. Gene therapy

Answer:- {B} Genetic engineering  

18. The expression of a transgene in the target tissue is identified by a

1. Transgene
2. Promoter
3. Enhancer
4. Reporter

Answer:- {D} Reporter  

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19. ———– is used as a vector for cloning into higher organisms

1. Retrovirus
2. Baculovirus
3. Salmonella typhimurium
4. Rhizopus nigricans

Answer:- {A} Retrovirus  

20. Which bacterium is used in the production of insulin by genetic engineering?

1. Saccharomyces
2. Rhizobium
3. Escherichia
4. Mycobacterium

Answer:- {C} Escherichia